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KMID : 0385520120250040250
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Analytical Science & Technology
2012 Volume.25 No. 4 p.250 ~ p.256
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Establishment of content criteria of marker compounds through the monitoring of Achyranthis Radix collected from Korea and China
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Kim Dae-Hyeon
Kim Sang-Hyuk
Shin Min-Chul
Chu Van-Men
Kang Jong-Seong
Jang Yu-Seon
Lee Young-Keun
Woo Mi-Hee
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Abstract
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Two marker compounds of Achyranthis Radix, ecdysterone and inokosterone, were analyzed by HPLC on an ODS column (250 × 4.6 mm, 5 μm) with a mobile phase of 15% acetonitrile containing 0.08% formic acid at a flow rate of 1.0 mL/min and a detection wavelength of UV 254 nm. The method was validated by ICH guideline and applied to the monitoring of marker compounds in 93 samples of Achyranthis Radix collected at various areas in Korea and China. The new content criteria of ecdysterone and inokosterone, established using linear regression method were 0.033% and 0.020%, respectively. When the new content criteria were applied to the quality control test of commercial Achyranthis Radix, 95.4% of total samples including 100% of Korean and 92.6% of Chinese samples were passed the test. Application of new content criteria could protect the Korean products and decrease the distribution of Chinese products with lower quality.
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KEYWORD
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content criteria, ecdysterone, inokosterone, Achyranthis Radix, HPLC
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